Cellular Repair Protocol
A 12-week stacked protocol targeting mitochondrial repair, DNA/telomere protection, neuroprotection, and longevity signaling.
Important: This content is for informational and research purposes only and is not intended for human or veterinary use. Consult your physician for health-related guidance and laboratory monitoring. Any health or longevity protocol assumes diet and exercise are already optimized.
Protocol Overview
This 12-week stacked protocol targets mitochondrial repair (SS-31, NAD+), DNA/telomere protection (Epithalon), neuroprotection (Pinealon), and longevity signaling (FOXO4-DRI). Administer subcutaneously (subQ) daily except where noted. Cycle: 8 weeks on, 4 weeks off. Monitor via bloodwork (liver/kidney function, inflammation markers). Hydrate well; combine with exercise/fasting for synergy.
Dosing Summary
| Peptide | Dosage | Frequency | Cycle | Notes |
|---|---|---|---|---|
| SS-31 | 500 mcg | Daily subQ (AM) | 8 weeks on / 4 off | Reconstitute 5mg vial with 1ml BAC water |
| Pinealon | 100–200 mcg | Daily subQ (PM) | 10–20 days on / 1–2 months off | Short cycles; brain repair focus. 20mg vial in 2ml water |
| Epithalon | 5–10 mg | Daily subQ (PM) for 10–20 days | 10–20 days on / 4–6 months off | Telomere extension; repeat 2–3×/year. 10mg vial in 2ml water |
| NAD+ | 50–150 mg | Daily subQ (AM) | 4–8 weeks on / 4 off | Energy boost; nasal/sublingual alt. 500mg vial in 5ml water |
| FOXO4-DRI | 5–10 mg | 3×/week | 1–2 weeks, 3×/year | Senolytic; use 3 days/week to avoid tolerance. 10mg vial |
Stack Schedule Example (Weeks 1–8)
- AM: SS-31 + NAD+
- PM: Pinealon (first 20 days) + Epithalon (first 20 days) + FOXO4-DRI (Mon/Wed/Fri)
Rotate off individually post-cycle to assess tolerance.
Peptide Details
1. SS-31 (Elamipretide)
SS-31 is a cell-permeable tetrapeptide (D-Arg-Dmt-Lys-Phe-NH2) that selectively partitions into the inner mitochondrial membrane with ~5000-fold enrichment via high-affinity electrostatic binding to cardiolipin (CL), an anionic diphospholipid essential for cristae structure and electron transport chain (ETC) function.
Key effects:
- Stabilizes CL against peroxidation by inhibiting cytochrome c peroxidase and scavenging H2O2/ROS
- Optimizes ETC supercomplexes (enhances CI–CIV coupling for ATP production)
- Prevents mPTP opening and mitochondrial fragmentation by balancing OPA1 isoforms
- Re-energizes ATP flux, reduces ROS, protects cristae integrity in ischemia, aging, and disease
2. Pinealon
Pinealon (Glu-Asp-Arg tripeptide) modulates pineal gland activity, upregulating neurotrophic factors like BDNF/NGF to promote neuronal survival, synaptic plasticity, and DNA repair in brain cells under oxidative/traumatic stress. It reduces neuroinflammation and enhances cognitive recovery via gene expression changes favoring neuroprotection.
3. Epithalon (Epitalon)
Epithalon (Ala-Glu-Asp-Gly tetrapeptide) induces telomerase activity (hTERT upregulation), elongating telomeres and stabilizing chromosomes against replicative senescence. It normalizes pineal melatonin synthesis, suppresses oxidative damage/p53-mediated apoptosis, and reprograms epigenetics for anti-aging effects like immune modulation and lifespan extension.
4. NAD+ (Nicotinamide Adenine Dinucleotide)
NAD+ acts as a critical cofactor for sirtuins (SIRT1/3/6), PARPs, and mitochondrial dehydrogenases. It fuels deacetylation of histones/peroxisome proteins for DNA repair, autophagy, and circadian regulation; restores depleted mitochondrial NAD pools to boost ETC efficiency (complex I activity), ATP synthesis, and ROS detoxification via redox shuttling.
5. FOXO4-DRI
FOXO4-DRI (D-retro-inverso peptide) selectively disrupts the FOXO4-p53 nuclear interaction in senescent cells, displacing p53 from DNA-binding sites. This triggers caspase-mediated apoptosis in irreparably damaged (senescent) cells while sparing healthy ones, reducing senescence-associated secretory phenotype (SASP) inflammation and promoting tissue rejuvenation and stem cell niches.
Disclaimer: This protocol is for educational and research purposes only. It is not medical advice. These products are not approved by the FDA. Consult a qualified healthcare provider before starting any new protocol.